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. 2015 Aug 4;7(8):4369–4384. doi: 10.3390/v7082821

Figure 4.

Figure 4

H7N9 specific T cell responses evaluated by IFN-γ ELISPOT assay. Mice were immunized with H7N9 VLPs, virions or PBS (n = 8 mice/group) for three times at two-week intervals. Spleen cells were collected from individual mouse and stimulated in vitro with H7N9 virions. ELISPOT assay was performed to test IFN-γ PRODUCTION. The mean number of spot forming cells/106 splenocytes was shown with a S.D. bar. * indicates p < 0.05; ** indicates p < 0.01.