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. 2015 Aug 4;7(8):4369–4384. doi: 10.3390/v7082821

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Serum neutralizing antibody titer of vaccinated mice. BALB/C mice were immunized with H7N9 VLPs, virions and PBS (n = 8 mice/group) for three times at two weeks interval. The mice were sacrificed at day 42 and the serum neutralizing antibodies specific to A/Wuxi/1/2013(H7N9) (A); A/Wuxi/2/2013(H7N9) (B); A/Nanjing/6/2013(H7N9) (C); A/Suzhou/3/2013(H7N9) (D); A/Jiangsu/1/2009 (H1N1) (E) and A/Nanjing/1/2009 (H3N2) (F) viruses were assessed by microneutralization assay. The cytopathic effect (CPE) was observed to determine neutralization titers at day 7. Data from each group were expressed as geometric mean titer (GMT) with a S.D. bar. * indicates p < 0.05; ** indicates p < 0.01. H7N9 virions, VLP and PBS: H7N9 virions, H7N9 VLPs, and PBS vaccinated mice serum.