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Schematic of native HPV virion production. HPV-positive cells are seeded on top of collagen plugs comprised of collagen and J2 3T3 feeder cells. Once the cells are have grown to confluence, the plugs are lifted onto a support grid. The rafts are then fed via diffusion from media underneath the grid. After 10–20 days, the tissue is harvested, homogenized, and benzonase treated to get a final viral preparation.
