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ELISPOT responses for IFN-γ, IL-2, IL-4, and IL-17. PBMC from 45 donors were plated at 250,000 cells/well and 50 μg/mL of HCMV antigen was added to induce a recall response for INF-γ (blue bars), IL-2 (red bars), IL-4 (green bars), and IL-17 (purple bars) from CD4 cells. SFU for each cytokine was established. SFU greater than 100 spots per well are not shown here. Raw data is available as part of Table S1.
