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Establishing optimal antigen dose for HCMV-specific CD4 cell stimulation. PBMC were plated at 250,000 cells per well and was stimulated with different concentrations of HCMV antigen starting from 100 μg/mL to 0.01 μg/mL. SFU recorded from IFN-γ, IL-2, IL-4, and IL-17 ELISPOT assays are shown for each of these antigen concentrations.
