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. 2015 Aug 6;7(8):4414–4437. doi: 10.3390/v7082828

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Longitudinal studies of donors show similarity in responses over multiple years. PBMC from six different donors were isolated at different time points over years. Some donors were tested multiple times within 1 year (Donor ID 13, 41, 44), others within 3 years (ID 43, 45) and one donor was tested over 5 years (ID 42). The dates at which PBMC was isolated is specified in the figure for each donor. PBMC isolated from each donor at the different time points, at 250,000 per well, was activated with 50 μg/mL HCMV antigen and the cytokine response for IFN-γ, IL-2, IL-4, and IL-17 was determined. Mean and SD of SFU from three repetitive wells are shown.