Figure 6.

Induction of autophagy with rapamycin enhanced the replication of BTV1. (A) Effects of 100 nM rapamycin treatment on LC3-II accumulation and VP2 or NS3 expression. The intensity ratios of proteins relative to β-actin are presented below the blots; (B,C) Representative results are shown with graphs representing the intensity of LC3-II/actin or VP2/actin bands; (D) The titers of BTV1 produced by rapamycin-treated BSR cells. The virus yields were shown as TCID₅₀/mL at 24 hpi and 36 hpi. Results represent the mean ± SD of triplicate experiments. * p < 0.05 and ** p < 0.01, significantly different; ns, no significant difference.