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Pharmacological or siRNA treatments had no impact on cell viability. Cell viability was detected by WST-1 assay after treatments with 3-methyladenine (3-MA), chloroquine (CQ), rapamycin (Rapa), or transfection with siBeclin1 for 48 h. Light absorption at 450 nm was recorded and expressed as percentage of relative cell viability, the values represented mean ± SD. “ns” means no significant difference, p > 0.05.
