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Density of bands from RT-PCR in each lane normalized to the total RNA as determined by the density of bands in RT-PCR for β -actin. Assuming β -actin was 4 562.39 units (pixels of brightness), the calculation [4 562.39/(density of actin)] × (density of each gene) equals each gene normalized to β -actin. Data shown are representative of three separate experiments. aP < 0.05.
