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. 2004 Aug 15;10(16):2344–2351. doi: 10.3748/wjg.v10.i16.2344

Figure 9.

Figure 9

A,B,C: Freshly isolated PSCs were incubated with 50 mL/L serum (panel A), HNE (at 1 µmol/L, panel B), or serum-free medium (panel C) for 7 d. Morphological changes characteristic of PSC activation were assessed after staining with GFAP. Original magnification: × 10 objective. D: Total cell lysates (approximately 25 µg) were prepared from cells treated with serum-free medium (“Cont”), 50 mL/L serum (“FBS”), or HNE (at 1 µmol/L) for 7 d, and the levels of α -SMA and G3PDH were determined by Western blotting.