Figure 1.
Design of green-inside red-outside (GIRO) labeling (A) schematized paradigm for dye additions. MG-BTau is added first to label surface-exposed FAP; MHN-Ester is then added to label all remaining sites to produce a surface-red internal-green fluorescent signal pattern. (B) Schematic of dL5** FAP, cell excluded MG-BTau, and cell permeable MHN-Ester. Structure shown is dimer of L5** FAP,33 dL5** is formed by addition of a G4S peptide linker. (C) Condensed schematic of MHN-Ester synthesis (see Supporting Information for detailed synthesis).