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. 2015 Aug 24;26(9):1963–1971. doi: 10.1021/acs.bioconjchem.5b00409

Figure 4.

Figure 4

Two-color labeling tracks changes in relative surface expression. (A) Representative fluorescence intensity histograms for Brefeldin A (BFA) treated cells over 18 h. (B) Median fluorescence values for labeled samples for surface (MG-BTau) and internal (MHN-Ester). The normalized relative surface expression (MG-Btau/MHN-Ester) is shown as a measure of surface residence. (C) RSE declines to 77.7% after 6 h, and to 38.4% after 18 h. Error bars are 95% confidence intervals, n = 9–12 replicates per condition from 4 experiments for all conditions except for 18 h, for which n = 9 from 3 experiments. (D) Representative fluorescence intensity histograms for dynasore treated cells over 6 h. (E) Quantification of surface and internal labeling in dynasore treated cells. Median fluorescence values were averaged across samples. (F) RSE of dynasore treatment shows a steady increase to 127.0% after 6 h (n = 9 biological replicates from 3 experiments). Error bars are 95% confidence intervals, one-way ANOVA with Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.