Table 1. Properties of dL5** FAP Fluorogen Activationa.
λmax | λex | λem | εmax | Φ | εB/εFb | ΦB/ΦF | ARc | KD | |
---|---|---|---|---|---|---|---|---|---|
MHN-Ester | 422 | (422) | (488) | 5.9 × 104 | 4.2 × 10–4 | 12 | 710 | 8520 | 42.5 |
MHN-Ester/dL5** | 456 | 456 | 532 | 6.4 × 104 | 0.3 | ||||
MG-BTau | 606 | (606) | (636) | 9.1 × 104 | 9.5 × 10–5 | 3.5 | 2010 | 7035 | 18 |
MG-BTau/dL5** | 633 | 633 | 668 | 1.1 × 105 | 0.19 |
Units: λ, nm; ε, M–1 cm–1; KD, pM.
εB and εF: Extinction coefficients of free and bound dye at microscopy excitation wavelength (488 and 640 nm for MHN-ester and MG-BTau respectively).
Activation Ratio: (εB/εF) × (ΦB/ΦF).