. 2015 Aug 24;26(9):1963–1971. doi: 10.1021/acs.bioconjchem.5b00409

Table 1. Properties of dL5** FAP Fluorogen Activation^a.

	λ_max	λ_ex	λ_em	ε_max	Φ	ε_B/ε_F^b	Φ_B/Φ_F	AR^c	K_D
MHN-Ester	422	(422)	(488)	5.9 × 10⁴	4.2 × 10^–4	12	710	8520	42.5
MHN-Ester/dL5**	456	456	532	6.4 × 10⁴	0.3	12	710	8520	42.5
MG-BTau	606	(606)	(636)	9.1 × 10⁴	9.5 × 10^–5	3.5	2010	7035	18
MG-BTau/dL5**	633	633	668	1.1 × 10⁵	0.19	3.5	2010	7035	18

Units: λ, nm; ε, M^–1 cm^–1; K_D, pM.

ε_B and ε_F: Extinction coefficients of free and bound dye at microscopy excitation wavelength (488 and 640 nm for MHN-ester and MG-BTau respectively).

Activation Ratio: (ε_B/ε_F) × (Φ_B/Φ_F).

Table 1. Properties of dL5** FAP Fluorogen Activationa.

Table 1. Properties of dL5** FAP Fluorogen Activation^a.