Table 2. The discrimination ratios (DRs) of specific biotin-probes for their respective full-match over other single-base mismatch DNA targets. The data shown in the brackets are after background correction, error bars are the standard deviations of 3 parallel samples. DR is defined as the signal ratio of the full-match target, Pn/Tn, over their respective SNP targets, Pn/Tm (where n ≠ m).
| Target (base 17) | Probe |
|||
| P1 (G) | P2 (A) | P3 (T) | P4 (C) | |
| T1 (C) | 1 (1) | 35 ± 4.1 (43 ± 5.1) | 12 ± 0.4 (13 ± 0.4) | 91 ± 6.2 (298 ± 20) |
| T2 (T) | 53 ± 15 (70 ± 20) | 1 (1) | 30 ± 1.1 (36 ± 1.2) | 21 ± 1.4 (25 ± 1.7) |
| T3 (A) | 57 ± 16 (78 ± 22) | 76 ± 8.9 (133 ± 16) | 1 (1) | 5.2 ± 0.4 (5.4 ± 0.4) |
| T4 (G) | 96 ± 27 (176 ± 50) | 121 ± 14 (383 ± 45) | 27 ± 1.0 (32 ± 1.1) | 1 (1) |
| Blank | 212 ± 60 (—) | 175 ± 21 (—) | 162 ± 5.8 (—) | 130 ± 9 (—) |