Skip to main content
. 2015 Sep 18;6:8237. doi: 10.1038/ncomms9237

Figure 6. Mice with selective inhibition of PKA in AgRP neurons have reduced adiposity.

Figure 6

(a) EGFP-labelled Cre expression in the hypothalamus of Agrp-CreEGFP mice (left: scale bar, 400 μm) and tdTomato expression and immunofluorescent staining of POMC in the ARC of Agrp-Cre/tdTom mice (right: scale bar, 100 μm). (b) Representative images of pCREB(S133) immunostaining in the ARC of Agrp-Cre/tdTom and Agrp-Cre/tdTom/RIαB mice following a 24-h fast. Scale bars, 100 μm, n=3 mice for each genotype (c) Quantification of pCREB staining intensity (left) and ratio of AgRP neurons (as indicated by tdTomato) that express pCREB (right) in the ARC of Agrp-Cre/tdTom and Agrp-Cre/tdTom/RIαB mice as shown in b. N=3 mice for each genotype. (**P<0.01, ***P<0.001 by two-tailed Student's t-tests). (d,e) Representative immunohistochemistry (d) and quantification (e) of c-Fos expression in RIαB-Off (left) and RIαB-ON (right) mice after a 24-h fast in the ARC (scale bars, 200 μm) and PVN (scale bars, 100 μm) of the hypothalamus. N=3 for each genotype. (f) Body weight changes of Argp-Cre and RIαB-Off mice (n=9) and RIαB-On mice (n=6) on high-fat diet (HFD) from 6 week of age for 12 weeks. ***P<0.001 between genotypes by two-way analysis of variance. (g) Weight of major fat pads (gonadal, retroperitoneal and inguinal) of Argp-Cre mice, RIαB-Off mice and RIαB-On mice fed either on chow diet (n=5 per group) or HFD (as shown in f) at 18–19 weeks of age. Data are presented as mean±s.e.m. and analysed by two-tailed Student's t-test (*P<0.05, **P<0.01, ***P<0.001).