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Immunostaining images of outgrown cells at Day 3 and Day 7: (a, b) ALP, (c) MLO-A5 cells used as ALP-positive control, (d, e) sclerostin, and (f) normal goat IgG as negative control. Nuclei stained by DAPI (blue), ALP (red), and sclerostin (red). Scale bar: 50 µm.
