Fig. 1.

GRP stimulation results in RhoA activation in colon cancer cell lines. Time-course of RhoA activation in colon cancer cell lines in response to GRP stimulation. Caco-2 (A) and HT-29 cells (B) serum-starved overnight and then incubated with GRP for indicated time(s). Cell lysates were used for GST-RBD pulldown (see Materials and Methods). The precipitate and lysate samples were then used for Western blot to detect RhoA and GAPDH. GAPDH is used as loading control. Shown are representative images from three independent experiments. RBD, Rho binding domain.