[Table/Fig-1]:

Primers and their sequences for various antibiotic resistance encoding genes used in this study

Target genes	Primer sequences	Product (bp)	Annealing temper-ature (°C)	Reference
mecA	F: TGCTATCCACCCTCAAACAGG	286	54.5	Abimanyu et al.,[14]
	R: AACGTTGTAACCACCCCAAGA
femA	F: AAAAAAGCACATAACAAGCG	132
	R: GATAAAGAAGAAACCAGCAG
erm (A)	F: AAGCGGTAAACCCCTCTGA	190	55	Strom-menger et al., [6]
	R: TTCGCAAATCCCTTCTCAAC
erm (C)	F: AATCGTCAATTCCTGCATGT	299
	R: TAATCGTGGAATACGGGTTTG
tetK	F: GTAGCGACAATAGGTAATAGT	360
	R: GTAGTGACAATAAACCTCCTA
tetM	F: AGTGGAGCGATTACAGAA	158
	R: CATATGTCCTGGCGTGTCTA
msrA	F: GAAGCACTTGAGCGTTCT	287	50	Shittu et al., [7]
	R: CCTTGTATCGTGTGATGT
dfrA	F: CTCACGATAAACAAAGAGTCA	201
	R: CAATCATTGCTTCGTATAACG
aac(6′)-Ie aph(2″)	F: CATTATACAGAGCCTTGGGA	279	57	Ida et al., [8]
	R: AGGTTCTCGTTATTCCCGTA
ant(4′)-Ia	F: ATGGCTCTCTTGGTCGTCAG	367
	R: TAAGCACACGTTCCTGGCTG
aph(3′)-IIIa	F: CGATGTGGATTGCGAAAACT	175
	R: CACCGAAATAACTAGAACCC
fusB	F: TCATATAGATGACGATATTG	496	53	Castanheira et al., [10]
	R: ACAATGAATGCTATCTCGAC
fusC	F: GATATTGATATCTCGGACTT	128
	R: AGTTGACTTGATGAAGGTAT
fusD	R: TGCTTATAATTCGGTCAACG	525
	R: TGGTTACATAATGTGCTATC
mupA	F: TATATTATGCGATGGAAGGTTGG R: AATAAAATCAGCTGGAAAGTGTTG	456	57	Yun et al., [9]

All the PCR reactions were carried out Initial Denaturation:94°C- 4 minutes, Denaturation: 94°C- 1 minute, Annealing - refer table 1, Extension: 72°C- 1minute, Final extension 72°C- 7minutes.