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. 2015 Aug 1;9(8):DC06–DC09. doi: 10.7860/JCDR/2015/13962.6315

[Table/Fig-1]:

Primer sequences for nuc and mecA genes for PCR and LAMP assay

Genes Primer sequences
PCR
nucFP CAAAGCATCAAAAAGGTGTAGAGA
nucRP TTCAATTTTCTTTGCATTTTCTACCA
mecAFP GGCAATATTACCGCACCTCA
mecARP GTCTGCCACTTTCTCCTTGT
LAMP assay
nuc-F3 TCGCTTGCTATGATTGTGG
nuc-B3 ACATACGCCAATGTTCTACC
nuc-FIP GTACAGTTTCATGATTCGTCCCGCCATCATTATTGTAGGTGT
nuc- BIP TGTTCAAAGAGTTGTGGATGGTGTACAGGCGTATTCGGTT
nuc-FLP TTGAAAGGACCCGTATGATTCA
nuc-BLP GATACGCCAGAAACGGTGA
mecA-F3 GGTACAAGATGATACCTTCGTT
mecA-B3 ATAGCAGTACCTGAGCCAT
mecA-FIP TCTTCAGAGTTAATGGGACCAAACAGAAAGTCGTAACTATCCTC
mecA-BIP AAGCTCCAACATGAAGATGGCTTGTATGTGCGATTGTATTGC
mecA-FLP ACCTAATAGATGTGAAGTCGCT
mecA-BLP CGTGTCACAATCGTTGACG

FP-Forward primer

RP-Reverse primer

F3-Forward outer primer, B3-Backward outer primer

FIP-Forward inner primer, BIP-Backward inner primer

FLP-forward loop primer, BLP-backward loop primers