Fig. 2.
Effect of HU-444 on TNF-α production in vitro and in vivo. (A) TNF-α (determined by ELISA) production by TG macrophages following incubation with 1 μg/ml LPS in presence of HU-444 for 24 hours. Control macrophages (105) produced 59 ± 3 pg/ml TNF-α during the 24 hours. *Statistically significant difference with a P value ≤0.05. (B) TNF-α in mice sera 90 minutes after injections of LPS and HU-444. TNF titer in the control LPS sera was 732 ± 60 pg/ml. *Statistically significant difference with a P value ≤0.05.