TABLE 2.
Xenobiotic-induced cytotoxicity in LLC-vector and LLC-MDR1-WT cells
| LLC-PK1 Cellsa | EC50 Values (95% Confidence Interval) |
|
|---|---|---|
| LLC-Vector Cellsb | LLC-MDR1-WT Cellsb | |
| Doxorubicin (nM)c | 32.2 (13.7–85.6) | 1978 (1015–3850)*** |
| + GF120918c | 24.8 (12.0–51.3) | 8.06 (1.32–49.2)††† |
| + Verapamilc | 43.4 (12.0–155.9) | 268 (90.7–98.6)††† |
| Colchicine (nM)c | 13.3 (N.D.)d | 645 (467–892) |
| + GF120918c | 12.6 (10.8–14.5) | 17.5 (12.0–25.4)††† |
| + Verapamilc | 12.8 (N.D.)d | 179 (117–273)††† |
| Ivermectin (µM) | 4.86 (4.51–5.23) | 4.04 (3.76–4.35)*** |
| + GF120918 | 5.76 (5.33–6.23)†† | 4.59 (3.99–5.28) |
| + Verapamil | 3.99 (3.31–4.81)† | 4.25 (4.02–4.50) |
| Diazinon (µM) | 1789 (N.D.)d | 384 (279–528) |
| + GF120918 | 132 (68.1–255) | 414 (165–1040) |
| + Verapamil | 592 (50.6–6920) | 373 (159–877) |
| Dieldrin (µM) | 51.4 (41.1–64.4) | 75.4 (62.9–90.4)* |
| + GF120918 | 38.2 (28.0–52.0) | 70.7 (57.0–87.6) |
| + Verapamil | 33.0 (27.3–39.8)† | 40.0 (32.4–49.4)††† |
| Endosulfan (µM) | 14.0 (10.4–18.8) | 19.4 (12.5–30.2) |
| + GF120918 | 17.4 (11.6–26.0) | 40.7 (16.9–98.0) |
| + Verapamil | 8.40 (5.01–14.1) | 16.8 (10.7–26.4) |
| Maneb (µM) | N.D.d | 40.3 (32.7–49.6) |
| + GF120918 | 24.6 (13.9 – 43.7) | 37.7 (30.0–47.3) |
| + Verapamil | N.D.d | 39.2 (32.9–46.7) |
| MPP+ (µM) | 27.1 (16.7–43.9) | 418 (170–1030)*** |
| + GF120918 | 84.9 (37.1–194) | 566 (299–1070) |
| + Verapamil | 31.9 (18.1–56.2) | 69.0 (39.4–121)†† |
| Rotenone (nM) | 12.5 (7.9–19.8) | 27.7 (21.7–35.3)** |
| + GF120918 | 17.4 (12.7–23.8) | 35.0 (23.5–52.1) |
| + Verapamil | 5.89 (3.97–8.73) | 7.24 (4.50–11.6)††† |
N.D., not determined.
a
Significant differences between cell types in the xenobiotic treatment alone group; *P < 0.05, **P < 0.01, and ***P < 0.001.
b
Significant differences within a cell type between the xenobiotic treatment alone group and in the presence of a P-gp inhibitor; †P < 0.05, ††P < 0.01, and †††P < 0.001.
c
Doxorubicin and colchicine have been reported previously (Lacher et al., 2014).
d
Nonlinear regression was unable to estimate confidence intervals.