Table 2.

Biased Distribution of Genes with Nonsynonymous Mutations in Bacteria Evolved with Enhanced GroEL Levels (pgroE).

Generation	GO Classificationa	Enriched GO Categoryb	Probabilityc
1.8K	Biological processes	• Single organism process	1.25 × 10−4
		• Single organism cellular process	2.39 × 10−2
		• Energy derivation by oxidation	7.35 × 10−3
		• Cellular respiration	2.53 × 10−3
	Molecular functions	• Cation transmembrane transporter	6.54 × 10−3
		• Anion activity	1.15 × 10−2
		• Ion activity	2.45 × 10−2
		• Substrate specific transmembrane transporter activity	4.18 × 10−2
		• Carbohydrate derivative activity	5 × 10−2
	Cellular components	• Cellular component	4.61 × 10−3
		• Membrane	8.74 × 10−3
		• Plasma membrane	1.17 × 10−2
		• Cell part	1.60 × 10−2
		• Cell	1.60 × 10−2
		• Cell periphery	1.97 × 10−2
4K	Biological processes	• Single organism process	3.65 × 10−5
		• Single organism cellular process	1.86 × 10−2
		• Cellular respiration	1.68 × 10−2
		• Energy derivation by oxidation	4.82 × 10−2
	Molecular functions	• Ion binding	1.99 × 10−3
		• Catalytic activity	7.34 × 10−3
		• Anion binding	2.02 × 10−2
		• Molecular function	3.63 × 10−2
	Cellular components	• Cellular component	3.92 × 10−3
		• Membrane	1.33 × 10−2
		• Plasma membrane	3.48 × 10−3
		• Cell part	7.57 × 10−3
		• Cell	7.57 × 10−3
		• Cell periphery	1.17 × 10−2
		• Oxidoreductase complex	4.53 × 10−2

^aGO major categories.

^bGO categories enriched for genes with nonsynonymous SNPs.

^cProbability of enrichment of GO categories under a hypergeometric test corrected for multiple testing using Bonferroni correction.