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. Author manuscript; available in PMC: 2016 Sep 10.
Published in final edited form as: Neuroscience. 2015 Jul 15;303:604–629. doi: 10.1016/j.neuroscience.2015.07.026

Fig. 14.

Fig. 14

Distribution of Cx36 and EGFP around the periphery of the LSO. (A) Immunofluorescence labelling of Cx36 in adult mouse, showing Cx36 localized around the peripheral margins of the LSO (arrows), with the LSO itself largely devoid of labelling (asterisk). (B) Images of the lateral margin of rat LSO, showing large Nissl counterstained neurons in the region (B1, arrows) and labelling of Cx36 along this margin (B2, arrows). (C) Magnification of the boxed areas in (B) with labelling for Cx36 alone (C1) and after overlay with blue Nissl counterstain (C2), showing punctate appearance of labelling for Cx36 and association of Cx36-puncta with most of the relatively large periolivary neuronal somata (arrows). The inset in C2 shows co-localization of Cx36-puncta with vglut1-positive terminals on neuronal somata in the periolivary region. (D) Immunofluorescence labelling of EGFP and Cx36 at the peripheral margins of the LSO in EGFP-Cx36 mouse, showing weak labelling for EGFP within large neuronal somata along the lateral and ventral LSO margins (D1, arrows) and, in the same field, labelling for Cx36 along these margins (D2, arrows), where Cx36 is localized to EGFP-positive neurons, as shown in overlay (D3, arrows). The LSO itself (outlined by dotted line) shows only background green and red fluorescence.