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. 2015 Sep 14;210(6):991–1002. doi: 10.1083/jcb.201502029

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© 2015 Zhou et al.

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Figure 6. — Sortilin and PSAP comprise two independent and complementary pathways for PGRN lysosomal targeting. (A) Immunoblot for sortilin with lysates prepared from fibroblasts and N2a cells. Gapdh was used as a loading control. (B) Immunostaining for PGRN, LAMP1, and PSAP in fibroblasts derived from wild-type and Sort1^−/− mice. (C) Ectopic expression of sortilin in PSAP^−/− fibroblasts rescues the PGRN trafficking defect. PSAP^−/− fibroblasts were transfected with GFP-sortilin. 48 h after transfection, the cells were fixed and stained with sheep anti–mouse PGRN and rabbit anti–Cathepsin D. Representative images from three replicated experiments are shown. Bars: (main) 20 µm; (inset) 5 μm.