Table 1. Extract depletion conditions.

Antibody	Antibody/100-µl beads	Antibody beads to deplete 50 µl extract
	µg	µl
Bub1	10	100
BubR1	10	100
CENP-C	1.8	16.7
CENP-E	10	100 (2 rounds of 50)
Haspin	7	100 (2 rounds of 50)
INCENP	10	100
Mad1	10	100 (2 rounds of 50)
Sgo1&2	16 each	200 (2 rounds of 100)