Table 3. Antibodies used in this study.

Reactivity	Epitope	IF condition	WB condition	Species	Reference or source
	aa
Xenopus
Bub1	274–467	5 µg/ml	1.5 µg/ml	Rabbit	This study; Sharp-Baker and Chen, 2001
BubR1	189–369	5 µg/ml	1.5 µg/ml	Rabbit	This study; Chen, 2002
CENP-A	1–50	1.5 µg/ml	ND	Rabbit	This study; Maddox et al., 2003
CENP-C	207–296	1 µg/ml	1 µg/ml	Rabbit	Milks et al., 2009
CENP-E	826–1,106	2 µg/ml	ND	Rabbit	Milks et al., 2009
CENP-E	2,397–2,954			Rabbit	T. Lorca; Wood et al., 1997; Vigneron et al., 2004
CENP-K	276–287	2 µg/ml	ND	Rabbit	Milks et al., 2009
CENP-T	1–603	0.3 µg/ml	0.3 µg/ml	Rabbit	A.F. Straight
Dasra A	284–296	1.4 µg/ml	7 µg/ml	Rabbit	Sampath et al., 2004)
dynein		Undiluted hybridoma supernatant	ND	Mouse	A.F. Straight
H2A_T120ph		1:100	ND	Rabbit	Abcam (ab111492)
H3_T3ph		ND	1:5,000	Rabbit	EMD Millipore (07-424)
Mad1	FL	1:200	ND	Rabbit	R.H. Chen; Chen et al., 1998
Mad1	1–12			Rabbit	T. Lorca; Vigneron et al., 2004
Mad2	FL	1:500	ND	Rabbit	Y. Mao; Chen et al., 1996; Mao et al., 2005
Mps1	1–14	1:200	ND	Rabbit	Y. Mao; Abrieu et al., 2001
Mis12	FL	1:500	1:500	Rabbit	Emanuele et al., 2005
Ndc80	FL	1:500	1:1,000	Rabbit	McCleland et al., 2003
α-Tubulin		ND	1:20,000	Mouse	Sigma-Aldrich (T9026)
Xorbit	799–1,457	1:4,000	ND	Rabbit	R. Heald; Hannak and Heald, 2006
Zwint	FL	1:500	1:1,000	Rabbit	Emanuele et al., 2005
Human
ACA	CENP-A,B,C	1:800	ND	Human	Antibodies, Inc. (15-235)
BubR1		1:1,000	ND	Rabbit	EMD Millipore (MAB3612)

IF, immunofluorescence; WB, Western blotting.