Table 1. Embryonic viabilityof klp-7(−) mutants.
| Allele | Homozygote embryonic viability (15°C) | Homozygote embryonic viability (26°C) | Heterozygote embryonic viability (26°C) |
|---|---|---|---|
| N2 | 99% (n = 358) | 97% (n = 197) | |
| klp-7(or1092) | 90% (n = 323) | 0.1% (n = 1,530) | 99% (n = 408) |
| klp-7(or1292) | 93% (n = 318) | 0.8% (n = 367) | 97% (n = 604) |
| klp-7(tm2143) | 46% (n = 315) | 0.8% (n = 247) | |
| klp-7(RNAi) | 1% (n = 164) | ||
| Genotype | Embryonic viability (26°C) | ||
| klp-7(or1092)/klp-7(or1292) | 2% (n = 238) | ||
| klp-7(or1092)/klp-7(tm2143)) | 1% (n = 517) | ||
| klp-7(or1292)/klp-7(tm2143)) | 0.5% (n = 220) | ||
| klp-7 Rescue | |||
| klp-7(or1092) unc-68(e587) + gfp::klp-7 | 94% (n = 16) | ||
| klp-7(or1092) unc-68(e587) | 1% (n = 257) |
Embryonic viability (percent hatching) was scored for the wild type and each TS mutant at the permissive temperature (15°C) and after L4 upshifts to the restrictive temperature (26°C). Embryonic viability from heterozygous mutants, after L4 upshifts to the restrictive temperature, was scored to determine whether the mutations are recessive or dominant. Complementation tests also were scored after L4 upshifts of outcross F1s to the restrictive temperature.