FIG. 2.
Time course of HIV-1 RNA and HIV-1 p24 accumulation in supernatant fluids from colorectal explants infected with HIV-1BaL (A) or HIV-1CH077 (B). Twenty biopsy tissues from each of four HIV-1-negative study participants were infected with HIV-1BaL or HIV-1CH077 as described in Materials and Methods. Supernatant fluids were collected from the explant tissues at days 1, 2, 4, 7, 11, and 14 after infection at which time the tissue culture medium was replenished. Viral RNA was extracted from supernatant fluid and measured by qRT-PCR using two assays (Assay 3 and Assay 4). HIV-1 p24 levels were quantified by ELISA. Data are presented as means and standard error and samples assays were conducted in triplicate.