FIG. 3.
Time course of HIV-1 accumulation after infection with HIV-1BaL (A) or HIV-1CH077 (B) by assay. Twenty biopsy tissues from each of four HIV-1-negative study participants were infected with 1 × 105 HIV-1BaL or HIV-1CH077 as previously described. HIV-1 nucleic acid accumulation in tissue was measured by five different quantitative real-time PCR assays for HIV-1 nucleic acids and data are plotted according to assay type. Two assays were performed to detect HIV-1 provirus (Assay 1 and Assay 2) while two assays detected HIV-1 RNA (Assay 3 and Assay 4). A qualitative Alu-gag PCR method assessed HIV-1 DNA integration. The assays performed on collected supernatant fluids were qRT-PCR for HIV-1 RNA (Assay 3 and Assay 4) and HIV-1 p24, measured by ELISA. T-DNA, tissue HIV-1 DNA; T-RNA, tissue HIV-1 RNA; S-RNA, supernatant HIV-1 RNA. Assay units are the same as those presented in Figs. 1 and 2.