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. 2015 Oct 1;31(10):981–991. doi: 10.1089/aid.2015.0165

FIG. 4.

FIG. 4.

(A) Scatterplot matrix of tissue assays. A scatterplot depicting the relationships among all five qRT-PCR assays in tissue is shown. Two qRT-PCR assays for provirus were performed to assess levels of HIV-1 DNA (Assay 1 and Assay 2) and two qRT-PCR assays detected viral RNA (Assay 3 and Assay 4). HIV-1 DNA integration was detected by a qualitative, nested Alu-gag PCR assay. T-DNA, tissue HIV-1 DNA; T-RNA, tissue HIV-1 RNA; S-RNA, supernatant HIV-1 RNA. Assay units are the same as those presented in Figs. 1 and 2. (B) Scatterplot matrix of supernatant fluid assays. A scatterplot depicting the relationships among all three assays performed on supernatant fluid is shown. Two quantitative real-time assays detected HIV-1 RNA (Assay 3 and Assay 4) and HIV-1 p24 was determined by ELISA. S-RNA, supernatant HIV-1 RNA; S, supernatant HIV-1 p24.