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. 2015 Jul 14;16(9):1114–1130. doi: 10.15252/embr.201540514

Figure 6.

Figure 6

pS65-Ub is induced in mouse primary neurons upon mitochondrial damage

Mouse primary neuronal cultures were left untreated or challenged with valinomycin as indicated.

  1. Two representative images are shown for each condition. Cells were fixed and stained with anti-cyclophilin F (mitochondria, red), anti-pS65-Ub#2 (green), anti-TUJ1 (cyan), and Hoechst (blue). Scale bars, 10 μm.
  2. Quantification of the mean intensity of the pS65-Ub signal in (A).
  3. The pS65-Ub mask shown in black/white in (A) is a binary image that was generated to quantify the area of the pS65-Ub spots.

Data information: Analyses were performed on five images for each condition for three independent experiments (two-sided unpaired t-test; *P < 0.05). Shown are mean values ± SD.