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. 2015 Jul 3;16(9):1131–1144. doi: 10.15252/embr.201540298

Figure 3.

Figure 3

Usp5 sensitivity of S65 ubiquitin mutant chains

  1. Structure of ubiquitin in complex with Usp5 (PDB ID: 3IHP). S65 is highlighted in red on the cartoon representation of ubiquitin, while Usp5 surface is shown in gray.
  2. Anti-ubiquitin immunoblotting of in vitro generated mixed chains comprised of both WT and His-tagged S65E ubiquitin. Chains were subjected to disassembly with various concentrations of Usp5, and the abundance of the dimers was measured.
  3. Quantification of band signal of the Western blot shown in (B). Y-axis represents signal relative to the point of maximum signal for each series.