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. 2015 Jul 31;16(9):1145–1163. doi: 10.15252/embr.201540759

Figure 4.

Figure 4

Loss of BNip3 induces aerobic glycolysis and suppresses oxidative phosphorylation

  1. μPET/CT imaging of wild-type (n = 5) and BNip3 null (n = 5) mice at d50 following injection of mice with 18F-deoxyglucose to examine glucose uptake by tumors.
  2. Flow cytometric measurement of fluorescent glucose uptake by wild-type and BNip3 null MECs grown in vitro at 20% and 1% oxygen.
  3. Lactate output by wild-type and BNip3 null MECs grown at 20% and 1% oxygen was measured by NMR, performed in triplicate.
  4. Mass spectrometric measurement of total levels of glycolytic intermediates (n = 4 for each genotype).
  5. Mass spectrometric measurement of total levels of TCA cycle intermediates and of 13C-labeled intermediates after growth in [U13C]-glucose for 6 h (3 biological replicates and 3 experimental replicates).
  6. Oxygen consumption using Seahorse XF96 by wild-type (blue) and BNip3 null (red) MECs (6 experimental replicates per point), performed in triplicate experiments.
  7. Measurement of uptake of 14C-labeled glucose into nucleic acids in wild-type and BNip3 null MECs (performed twice in triplicate).
  8. Measurement of uptake of 14C-labeled glucose or glutamine into lipid in wild-type and BNip3 null MECs (performed twice in triplicate).

Data information: Results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.