Additional effects of BNip3 loss on metabolism

1. A Mass spectrometric measurement of total levels of glycerol-3-phosphate and serine plus the glycolytic intermediates from which they are generated.
2. B, C Oxygen consumption by wild-type and BNip3 null MECs grown in the presence of glutamine (B) or glucose plus glutamine (C).
3. D Oxygen consumption by BNip3 null MECs following expression of either empty vector (hashed line) or BNip3-expressing vector (solid line) (6 experimental replicates per point), performed in triplicate experiments.
4. E Mass spectrometric measurement of TCA cycle intermediates after 24 h of growth in [U-¹³C]-glutamine.
5. F Mass spectrometric measurement of total levels of amino acids in wild-type and BNip3 null MECs grown on glucose.

Data information: Results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.