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. 2015 Sep 2;16(9):1177–1191. doi: 10.15252/embr.201540467

Figure 3.

Figure 3

Sox2 has no influence on the binding of Oct6 to the Sox/Oct motif

  • A, B FP-EMSA analysis of titrations of GFP-Oct6, binding to the Nanog Sox/Oct oligonucleotide (10 nM) in the absence (A) or the presence (B) of 132 nM mCherry-Sox2 (n = 2). O6SD: GFP-Oct6-mCherry-Sox2 dimer, O6M: GFP-Oct6 monomer complex, FD: free DNA (n = 2).
  • C Plots for quantitation of aKd of GFP-Oct6 in the presence or absence of Sox2 are shown.

Source data are available online for this figure.