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. 2015 Sep 2;16(9):1177–1191. doi: 10.15252/embr.201540467

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© 2015 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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graphic file with name embr0016-1177-f9ev.jpg — Quantification of aK _d by FP-EMSA

Blue panel: DNA; green panel: protein detection. For quantification purpose, only the blue panel needs to be taken into consideration. The bound fraction was calculated according to the equation provided.

Sigmoid fitting function used to fit the bound fraction versus total protein concentration plot.

Source data are available online for this figure.