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. 2015 Sep 2;16(9):1177–1191. doi: 10.15252/embr.201540467

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Control experiments for the titration of Oct6 in the presence and absence of Sox2

  1. A Confirmative monomeric band was observed by studying proteins in the absence (−SO) or the presence (+SO) of the Nanog Sox/Oct sequence. Control is lysate from untransfected cells. dGdC is included as a non-specific competitor.
  2. B, C FP-EMSA analysis of titrations of GFP-Oct4, binding to the Nanog Sox/Oct oligonucleotide (10 nM) in the absence (B) or the presence (C) of 132 nM mCherry-Sox2.
  3. D Bound fractions observed from titrations shown in (B, C) were plotted against the respective concentration of Oct4 for quantifying the aKd of those titrations.

Data information: O4SD: GFP-Oct4-mCherry-Sox2 dimer complex, O6M: GFP-Oct6 monomer complex, S2M: mCherry-Sox2 monomer complex, O4M: GFP-Oct4 monomer complex, FO: free GFP-Oct4, FD: free DNA. n = 2. Source data are available online for this figure.