Figure 6.

microRNA changes associated with Pten-mediated tumorigenesis

1. Scheme to isolate Pten-deficient epithelial cells from mouse prostate. Prostates were isolated from 12-week-old transgenic mice expressing Probasin-Cre and the Rosa26^{lox-stop-lox-YFP} reporter allele plus and minus the Pten^flox/flox allele. YFP-positive cells were isolated using fluorescence-activated cell sorting technology. RNA was isolated and small RNA libraries were submitted for deep sequencing analysis.
2. Volcano scatter plot of log₂-fold difference between wild type and Pten^Δ/Δ versus log₁₀ P-values. microRNAs with differences > 2-fold and q-value < 0.05 are highlighted and labeled. Q-values were determined using Benjamini–Hochberg procedure.
3. Scatterplot of log₂ counts per million (cpm) expression values of subset of miRNAs with q-values < 0.05 and average counts in at least one group of > 100 cpm. The black bar represents the mean of the three values for each genotype.

Source data are available online for this figure.