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Significant molecular factors involved in the spleen with different age and proton irradiation comparisons (0 Gy adolescent (A), 0 Gy old (O), 0.5 Gy x 3 proton adolescent (A P), 0.5 Gy x 3 proton old (O P)). The mRNA expression determined by real-Time PCR (RTPCR) for MCM7, RUVBL2, CDK2, CD74 and TGFβ1 compared with the gene expression observed by Illumina. Log2 fold changes for mRNA were determined for each group, *P < 0.05 and **P < 0.01.
