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. 2015 Mar 31;212(8):1261–1269. doi: 10.1093/infdis/jiv202

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Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 5. — Expansion of M1-specific CD8⁺ T cells after in vitro stimulation. A, A representative histogram of the frequency of M1-specific CD8⁺ T cells after in vitro expansion in short (n = 9) and long (n = 13) telomere groups. Activation of M1-specific CD8⁺ T cells using monocyte-derived antigen-presenting cells (APCs), as described in “Participants, Materials, and Methods” section. B, Percentage of live M1-specific CD8⁺ T cells after 7-day in vitro stimulation by APCs plus M1 peptide. Circles represent results for single subjects; group means and standard errors of the mean are also shown. Live cells were identified based on the fluorescence-activated cell sorting profile with gating from forward and side scatter. C, Expansion of M1-specific CD8⁺ T cells after in vitro stimulation by APCs plus M1 peptide. M1-specific-CD8⁺ T cells were stained with M1 dextramer before and after culture and analyzed by means of flow cytometry. Population doublings (PDs) were calculated based on the live M1-specific CD8⁺ T cells before and after 7-day culture. Group means and standard errors of the mean are shown. *P < .05 (age adjusted).