Figure 5.

Butyrophilin-like 2–deficient (BTNL2^−/−) mice exhibit increased proportion and proliferation of T cells compared with wild-type (WT) mice during experimental cerebral malaria. WT or BTNL2^−/− chimeras mice were infected with 10⁶ Plasmodium berghei ANKA–infected red blood cells. After 6.5 days, spleens and brains were harvested, stained for indicated markers and analyzed by flow cytometry. A, Frequency of indicated T-cell subsets among CD45⁺ hematopoietic cells in spleen and brain. B, Representative dot plots of indicated T-cell subsets (CD4, regulatory T cells [Tregs], CD8) expressing the marker of proliferation Ki67. Numbers represent the percentages of Ki67⁺ cells in the gate shown. In all bar graphs, dots represent individual mice, and bar shows the mean. Data represent the pool of 2 replicate experiments (n = 5–6 mice). *P < .05; ^†P < .01; ^‡P < .0001 (unpaired t test); where values are not specified, differences are not significant.