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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Med Mycol. 2015 Apr 3;53(5):493–504. doi: 10.1093/mmy/myv012

Figure 3.

Figure 3

Amoebic predation of Cryptococcus. (A) The dynamics of amoebic predation of Cryptococcus wild-type strain H99, the znf2Δ mutant, and the PCTR4-2ZNF2 strain on V8 juice agar, V8+copper, and V8+BCS was recorded for 8 days. No expansion of predation zone on the cbk1Δ mutant was observed even after three weeks (not shown). (B) The area outside of the predation zone, at the front of the predation, and inside the predation zone of the PCTR4-2ZNF2 strain on V8+BCS agar medium. * indicate the yeast colonies. (C) From the predation zone of the PCTR4-2ZNF2 strain on V8+BCS agar medium, amoebae with attempts to phagocytose hyphae were observed (upper image) and amoebae with completely phagocytosed yeast cells were observed (lower image).