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. 2015 Sep 21;10(9):e0138798. doi: 10.1371/journal.pone.0138798

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© 2015 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Overexpression and purification of recombinant IifC. Cell extracts were prepared before (lane 1) and after (lane 2) IPTG induction. Recombinant IifC protein (lane 3) was further purified using Q-Sepharose High Performance anion-exchange column. (B) Enzymatic activity of IifC in the presence of indole in various pH buffers. pH dependent activity assay was carried out in 100 mM potassium phosphate buffer between pH 6.0 and 7.5, 100 mM Tris-HCl buffer between pH 8.0 and 9.5.