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. 2015 Sep 21;10(9):e0138344. doi: 10.1371/journal.pone.0138344

Fig 5. Extracellular Acidification Rate Following Isoproterenol Treatment.

Fig 5

The Seahorse XF Cell Mito Stress Test was performed on mature 3T3-L1 adipocytes following a 6 (A) or 48 hour (B) treatment with varying doses of isoproterenol (ISO). The last of 3 measurements was used to determine percentage of change in extracellular acidification rate (ECAR) within groups (n = 6 per treatment) and provides a measurement of glycolysis. The metabolic profile for both 6 (A) and 48 hours (B) was determined by plotting ECAR to OCR. The relationship between coupling efficiency with glycolysis (ECAR) was visualized following 6 and 48 hours of isoproterenol treatment by plotting both points against each other (C). Statistical differences compared to control are indicated with * p<0.05, ** p<0.01, and *** p<0.001.