Table 1. Primers used for Gene Expression Assays.
| Classification | Gene Symbol | Sense | Anti-Sense |
|---|---|---|---|
| Endogenous | ACTB | ACCTTCCAGCAGATGTGGAT | TAGAAGCACTTGCGGTGCACGA |
| control | GAPDH | AACAGCAACTCCCACTCTTC | CCTGTTGCTGTAGCCGTATT |
| Thermogenesis | UCP1 | CCTGGCAGATATCATCACCTTC | TGGTCCCTAGGACCTTTAT |
| PRDM16 | ACCTGCCACAGCAAAGAA | CCATCCAAGCAGAGAAGTAGAC | |
| Beige Markers | CD137 | GAGGTCAGAAGAGAAAGGGTTG | GTAGAGGACCCAGGTTTGATTC |
| CITED1 | AACCTTGGAGTGAAGGATCGC | GTAGGAGAGCCTATTGGAGATGT | |
| HOXC8 | TAGTGTTGGCGGAGGATTTAC | TTGATCCGCGCCGTATTT | |
| HOXC9 | TTGATCCGCGCCGTATTT | ACAGAACAATCAAGGCAGGT |
The above primers were designed using the PubMed database and IDT, Inc for qPCR. For the purpose of dCt calculations, ACTB was chosen as a better endogenous control. Abbreviations: beta actin (ACTB), glyceraldehyde 3-phosphatase dehydrogenase (GAPDH), cluster of differentiation 137 (CD137), Cbp/p300-interacting transactivator 1 (CITED1), homeobox protein C8 (HOXC8), homeobox protein C9 (HOXC9), uncoupling protein 1 (UCP1), PR domain containing 16 (PRDM16).