Fig. S5.

ΔNp63–HK2 axis is present also in HCC1937 breast cancer cell line. HCC1937 expresses a high level of ΔNp63. (A) ΔNp63 silencing results in ∼50% reduction in HK2 mRNA whereas HK2 protein is almost undetectable (B). (C) ChIP confirms that ΔNp63 physically binds in HCC1973 cells the enhancer region identified in the HK2 15th intron. Unlike normal epithelial cells, in cancer cells, HK2 has no preventive antioxidant effect as a result of its ADP-recycling activity, which is in line with the metabolic reprogramming occurring in cancer cells, in which aerobic glycolysis in preferred and there is reduction in mitochondrial respiration capacities. Cellular ROS (D, CM-DCFDA) and mitochondrial superoxide (E, MitoSOX Red) do not change upon introduction of siHK2. Interestingly, knockdown of HK2 results in a higher polarization of the mitochondrial membrane compared with the control (F). The data are the average of three independent experiments (mean ± SD; n = 3; *P < 0.01).