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Positional insertion of 13C-Ile to probe the structure of the small domain β-sheet of unliganded GCK. (A) Location of Ile insertions; (B–E) 1H-13C HMQC spectra of the L77I, L79I, L88I, and L146I variants of GCK demonstrate the appearance of new cross-peaks residing outside of the window expected for disordered isoleucines (boxed).
