Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Insertion of additional Ile probes is minimally perturbative as measured by steady-state kinetics
| Isoleucine variant | kcat, s−1 | K0.5, glucose, mM | Hill coefficient, H |
| Wild-type | 53 ± 1 | 8.5 ± 5.5 | 1.6 ± 0.1 |
| G446I | 40 ± 0.5 | 5.1 ± 0.5 | 1.3 ± 0.1 |
| V452I | 48 ± 0.7 | 6.9 ± 0.5 | 1.7 ± 0.2 |
| L463I | 58 ± 1 | 6.3 ± 0.4 | 1.6 ± 0.1 |
