Table S3.
Kinetic and thermodynamic characteristics of GCK variants
| Parameter | Wild-type | β-hairpin | α13-helix | α13 helix + β-hairpin |
| kcat, s−1 | 50 ± 0.5 | 84 ± 7.2 | 50 ± 2.0 | 86 ± 2.0 |
| K0.5, glucose, mM | 8.1 ± 0.4 | 1.6 ± 0.3 | 1.0 ± 0.1 | 0.14 ± 0.01 |
| Hill coefficient | 1.7 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 |
| Km, ATP, mM | 0.50 ± 0.01 | 1.1 ± 0.1 | 0.16 ± 0.01 | 0.70 ± 0.1 |
| KD, glucose, mM | 2.9 ± 0.2 | 8.7 ± 2.0 | 0.049 ± 0.02 | 0.056 ± 0.008 |
| kcat/K0.5, M−1⋅s−1 | (6.2 ± 0.1) x 103 | (5.3 ± 0.1) x 104 | (5.0 ± 0.1) x 104 | (6.1 ± 0.1) x 105 |