Table 1.

Proteolysis constants for wild-type and activated GCK variants

Protein	Kop (x 10−2)	ΔGc-u, kcal/mol
α13 helix	0.63 ± 0.05	3.00 ± 0.2
V455M	0.64 ± 0.06	2.99 ± 0.3
S64P	0.77 ± 0.07	2.89 ± 0.3
T65I	1.0 ± 0.1	2.73 ± 0.2
Wild-type	1.1 ± 0.08	2.70 ± 0.2
α13 helix + β-hairpin	1.2 ± 0.09	2.64 ± 0.2
M197V	1.7 ± 0.1	2.40 ± 0.2
β-hairpin	2.3 ± 0.2	2.23 ± 0.2
I211F	3.7 ± 0.3	1.95 ± 0.2
Y214C	5.1 ± 0.7	1.76 ± 0.2